Journal: JTCVS Open

Article Title: In vivo growing leporine model of pulmonary artery banding induces inflammation and vascular remodeling via pathways conserved across species

doi: 10.1016/j.xjon.2025.101560

Figure Lengend Snippet: Histological analysis of the fibrosis in response to an elevated PA gradient. A, Picrosirius red stain demonstrating dense fibrosis in the RV of a rabbit that underwent PAB. B, Picrosirius red stain demonstrating lack of fibrosis in the RV of the control rabbit. C, Masson's trichrome stain demonstrating dense fibrosis in the RV of a rabbit that underwent PAB. D, Masson's trichrome stain demonstrating relatively little fibrosis in the RV of the control rabbit. E, Masson's trichrome stain of the RV of a 2-month-old that had undergone bilateral banding of the branches of the PA, demonstrating prevalent fibrosis. F, Masson's trichrome stain of the RV from a 20-year-old with severe PAH, demonstrating prevalent fibrosis. G, Masson's trichrome stain of the RV from a 38-year-old with severe PAH, demonstrating prevalent fibrosis. H, Masson's trichrome stain of the RV from a 20-year-old otherwise healthy female brain-dead donor. Scales are 200 μm.

Article Snippet: Samples were stained using StatLab's Masson's Trichrome Stain Kit (StatLab, Cat: KTMTR EA).

Techniques: Staining, Control

Journal: bioRxiv

Article Title: The role of inflammation in a humanized mouse model of transthyretin cardiac amyloidosis

doi: 10.1101/2024.12.24.629308

Figure Lengend Snippet: (A-E) Representative Masson’s Trichrome staining (MTS) findings on endomyocardial biopsy of whole hearts and partial hearts (slides digitized at 20x) from male ATTR and control mice. (C) Masson’s trichrome staining shows that the connective fibrous tissue (arrows) is evidenced by intense blue staining, whereas amyloid deposits show a blue-violet color (asterisks). Original magnification, 40x. (F) Quantitative analysis of MTS for fibrosis and amyloid fibrils showed a significantly increased extent of amyloid and fibrosis in male ATTR hearts compared with controls. Each dot represents one field acquisition (magnification of 20x with a confocal microscope), 60 fields for 3 controls, and 39 for 3 ATTR mice. Data are presented as mean ± SEM. *p < 0.05 by nonparametric T-test.

Article Snippet: After deparaffinization and rehydration, the murine cardiac tissue was stained with Weigert’s iron hematoxylin, Biebrich scarlet acid fuchsin, and aniline blue from the Masson’s Trichrome Staining Kit (Polysciences, 25088).

Techniques: Staining, Control, Microscopy